اثر سیتوتوکسیک عصاره‌های آلی جلبک دریایی acerosa Gelidiella سواحل چابهار بر رده‌های سلولی سرطان سینه (MCF-7) و کولورکتال (HT-29)

Background and Objectives: Marine algae are one of the natural resources which produce a wide range of new secondary metabolites with various biological activities such&nbsp; as antitumor and anticancer properties. The present study was conducted in order to evaluate the invitro cytotoxic effect of marine algae Gelidiella acerosa organic extracts against breast (MCF-7) and colorectal (HT-29) cancer cell lines. Materials and Methods: In this laboratory study, cell lines of breast (MCF-7) and colorectal (HT-29) were cultured in RPMI medium with 10% fetal bovine serum (FBS). The cytotoxic effect of different concentrations (125, 250, 500 ,and 1000 &mu;g/ml) of algae extracts cultured cells were evaluated by MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide) and trypan blue assay. DNA fragmentation activity of the cells was evaluated by the 2% agarose gel after cell culturing by the different concentration of the algae extracts, trypsination ,and precipitation by RNAase. Data were analyzed using one-way ANOVA followed by Tukey post hoc test. Results: Survival of cancer cells in both methods was inversely correlated with the concentration and the percentage of survival decreased with increasing concentration. Concentration of 1000 &micro;g/ml of methanol extract showed the greatest effect compared with the controls (p<0.05). The methanolic extract LC50 (Lethal Concentration 50) on colorectal and breast cancer cells was 724.48&plusmn;25.52 and 845.36&plusmn;41.05 &micro;g/ml, respectively. Also, methanolic extract of algae was able to fragment the DNA of cancer cells and induce apoptosis dose dependent (p<0.05). Conclusion: In this study, results showed seaweed extract can&nbsp; be the basis for further studies in order to recognize the chemical compounds of the extracts. Also, after invivo, preclinical, and clinical studies the extract can be used in food and pharmaceutical industries. Key words: Cytotoxicity, MTT assay, Marine algae, Apoptosis, Breast cancer, Colorectal cancer &nbsp; Funding: This study was funded by the Iranian Science, Research ,and Technology Ministry for Research number 3.82601. Conflict of interest: None declared. Ethical approval: The Ethical Committee of Chabahar Maritime University approved the study. &nbsp; How to cite this article: Bavi Z, Ghaffari M, Taheri A, Soheili F. The Cytotoxic Effect of Chabahar Coast Marine Algae Gelidiella Acerosa Organic Extracts on Breast (MCF-7) and Colorectal (HT-29) Cancer Cell Lines. J Rafsanjan Univ Med Sci 2017; 16(8): 757-68. [Farsi]